Tumor-Promoting Effects of YAP1 and YAP S127A Overexpression in Prostate Cancer Cells

Authors : Filiz Kısaayak Çollak, Ümmühan Demir, Fatma Sağır
Pages : 354-360
Doi:10.7240/jeps.1743240
View : 77 | Download : 161
Publication Date : 2025-12-23
Article Type : Research Paper
Abstract :Understanding the molecular pathogenesis in cancer is crucial to shed light on novel anti-cancer treatments. YAP1 is important player in Hippo pathway to mediate tissue homeostasis. Aberrant activation of YAP1 has a role in cancer progression. This study aimed to understand the effect of overexpression of YAP1 and non-phosphorylated YAP S127A in prostate cancer progression. After transient overexpression of YAP1 and YAP S127A, the functional assays such as viability, 2D colony formation and soft agar colony formation were applied in LNCaP and PC3 cell lines. Additionally, subcellular localization of overexpressed YAP1 and YAP S127A was investigated. The cell viability was enhanced both in LNCaP and PC3 prostate cancer cell lines upon YAP S127A overexpression, with an increase of 33% in LNCaP and 25% in PC3 cells compared to control . 2D colony formation ability of PC3 cells were elevated in YAP S127A expressing cells compared to both YAP1 expressing cells (p = .037) and controls (p=.0003). The overexpressed YAP1 and YAP S127A were mainly localized to the cytoplasm. Although, the nuclear localization of overexpressed YAP1 and YAP S127A was limited, increased expression of them enhanced tumor promoting functions such as viability and 2D colony formation ability. Notably, these predominantly cytoplasmic YAP1 and YAP S127A despite the S127A mutation\\\'s design to resist phosphorylation and promote nuclear accumulation, suggest the presence of alternative regulatory mechanisms. Our results show that YAP1 may play a role in prostate cancer progression. Additional in vitro studies with more prostate cancer models and in vivo experiments are required to understand the biological impact of its cytoplasmic localization.
Keywords : prostat kanseri, hücre proliferasyonu, hippo sinyal yolağı, YAP1

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