Molecular detection of Theileria annulata in ixodid ticks in northwest of Iran

Authors : Ahmad NEMATOLLAHİ, İraj PANAHİ, Solin GHADERİ, Tina DANESHRAD

Pages : 55-55

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Publication Date : 2020-03-31

Article Type : Research Paper

Abstract :Ixodid ticks transmit various protozoan diseases in animals such as theileriosis that cause a lot of economic loss in livestock industry. The purpose of the present study was to detect Theileria annulata infection in ticks in cattle in Urmia district in North West of Iran. One hundred ticks were collected from some suspected flocks with history of theileriosis and were transported to laboratory. Taxonomic identification was done based on morphological characteristic. Ten tick species were found: Hyalomma anatolicum anatolicum insert ignore into journalissuearticles values(29%);, Hyalomma aegyptium insert ignore into journalissuearticles values(16%);, Boophyls anulatus insert ignore into journalissuearticles values(16%);, Dermacentor marginatum insert ignore into journalissuearticles values(14%);, Rhipicephalus bursa insert ignore into journalissuearticles values(9%);, Dermacentor niveus insert ignore into journalissuearticles values(4%);, Ornithodorus lahuransisinsert ignore into journalissuearticles values(4%);, Hyalomma anantolicum excavatuminsert ignore into journalissuearticles values(3%);, Rhipicephalus sanguinus insert ignore into journalissuearticles values(2%); and Haemaphysalis punctate insert ignore into journalissuearticles values(1%);.Salivary glands of ticks were isolated and examined using specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata insert ignore into journalissuearticles values(Tams-1); gene by PCR. Analysis of ticks by using PCR revealed T. annulata in 65insert ignore into journalissuearticles values(65%); of evaluated ticks. The high rate of infestation in ticks to T. annulata identifies the high risk of them to transporting the disease to cattle in North West of Iran and Hyalomma anatolicum anatolicum is the first candidate for transmitting of the disease.
Keywords : Theileria annulata, PCR