Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data

Gökmen Zafer PEKMEZCİ; Ertan Emek ONUK

doi:10.35229/jaes.738822

Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data

Authors : Gökmen Zafer PEKMEZCİ, Ertan Emek ONUK

Pages : 241-244

Doi:10.35229/jaes.738822

View : 49 | Download : 9

Publication Date : 2020-06-30

Article Type : Research Paper

Abstract :There is no study about the molecular characterization of the mitochondrial rrnS gene of Anisakis typica from the Turkish Mediterranean coasts. Thus, molecular characterization of the rrnS gene of A. typica from Turkish waters is aimed for the first time in the present study. Ascaridoid marine nematodes were collected and morphologically identified by light microscope. Identified Anisakis type I larvae were randomly selected and molecularly analysed by Restriction Fragment Length Polymorphism insert ignore into journalissuearticles values(RFLP); for the internal transcribed spacer insert ignore into journalissuearticles values(ITS); region. Anisakis typica larvae identified by PCR-RFLP analyses were amplified and sequenced with both directions for the rrnS gene. The rrnS sequence of A. typica from the Mediterranean coasts of Turkey insert ignore into journalissuearticles values(MT395672); showed 99.80% identity insert ignore into journalissuearticles values(100% query coverage); with rrnS gene of A. typica insert ignore into journalissuearticles values(JX500052); from the Caribbean Sea, Atlantic Ocean. Between A. typica from the Mediterranean Sea insert ignore into journalissuearticles values(MT395672); isolate and the Caribbean Sea isolate differed by only one nucleotide insert ignore into journalissuearticles values(C‒T, at alignment position 437);. Pairwise genetic distance between the rrnS sequence of A. typica herein and other A. typica from the Caribbean Sea was 0.002. Consequently, we determined the mitochondrial rrnS data of A. typica from the Mediterranean Sea in the present study for the first time. The valid genetic data insert ignore into journalissuearticles values(MT395672); reported here can be used to molecular identification of Anisakis species from the Mediterranean Sea and worldwide.
Keywords : Anisakis typica, Akdeniz, küçük alt ünite rRNA rrnS, moleküler karakterizasyon

ORIGINAL ARTICLE URL