Validation Methods for Phenolic Components with RP-HPLC-UV in Various Bee Products

Authors : Hilal Ebru ÇAKIR, Yakup ŞİRİN, Sevgi KOLAYLI, Zehra CAN

Pages : 13-19

View : 16 | Download : 8

Publication Date : 2018-04-01

Article Type : Research Paper

Abstract :In this study, a simple and a fast method were developed using RP-HPLC-UV for the separation and quantitative determination of phenolic compounds in various bee products. Fourteen phenolic compounds were identified using 70% acetonitrile in water, and 2% acetic acid in water as a mobile phase with a gradient elution mode. The validation method exhibited linearity insert ignore into journalissuearticles values(R 2 >0.994);, limits of detection insert ignore into journalissuearticles values(0.022-0.062 mg/L); and quantification insert ignore into journalissuearticles values(0.030-0.187mg/L);. Under the optimized conditions, 14 commercially available phenolic compounds were analyzed in less than 50 min. This method was successfully employed to study the phenolic profiles of bee products as well as other natural samples.     
Keywords : RP HPLC UV, phenolic compounds, bee products, quantification, validation