Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity

Authors : Ga DOS SANTOS, Aa LAGAREIRO NETTO, Pb MENDES, V NEGRÃO, MC MARCUCCI, SN DINIZ, MJ CARNEIRO, ACHF SAWAYA

Pages : 68-68

View : 11 | Download : 12

Publication Date : 2018-12-08

Article Type : Conference Paper

Abstract :Propolis, a resin produced by A. mellifera bees, presents several compounds of pharmaceutical interest and, further studies to demonstrate their pharmacological actions is sought. Capsules containing green propolis insert ignore into journalissuearticles values(BRPX); extracted in flax oil insert ignore into journalissuearticles values( 1 and 2 ); polyethylene glycol insert ignore into journalissuearticles values(PEG); insert ignore into journalissuearticles values( 3 and 4 ); were analyzed and analyzed according to IN 3 1 . The following parameters were evaluated: total phenols and flavonoids, quantification of Artepillin-C insert ignore into journalissuearticles values(HPLC); confirmed by UHPLC-MS, antitumor activity in MCF-7 insert ignore into journalissuearticles values(breast code 0162); and B16F10 insert ignore into journalissuearticles values(melanoma code 0046); and antimicrobial activity insert ignore into journalissuearticles values( E.coli , C. albicans and S .aureus );. The flavonoid content insert ignore into journalissuearticles values(%); of each capsule was 1 : 1.135 ± 0.009, 2 : 0.521 ± 0.039, 3 : 0.758 ± 0.020, 4 : 1.232 ± 0.09. The phenol content insert ignore into journalissuearticles values(%); of 1 : 2,509 ± 0,024, 2 : 2,280 ± 0,075, 3 : 1,039 ± 0,029, 4 : 3,426 ± 0,074. The content of Artepillin-C insert ignore into journalissuearticles values(mg / capsule); of 1 : 1.39 ± 0.12, 2 : 1.07 ± 0.05, 3 : 0.66 ± 0.01, 4 : 0.39 ± 0, 01. The IC50 insert ignore into journalissuearticles values(μg / mL); for MCF-7 was: 1 : 100.49 ± 6.41, 2 : 463.65 ± 0.71, 3 : 209.03 ± 35.99, 4 : 238.84 ± 4 , 16 and for B16F10, 1 : 277.53 ± 7.37, 2 : 357.48 ± 10.15, 3 : 376.15 ± 5.92, 4 : 286.87 ± 10.79. Inhibition halos ranged from 10 to 14 mm insert ignore into journalissuearticles values( E.coli );, 8 to 10 mm insert ignore into journalissuearticles values( C. albicans ); and 8 to 9 mm insert ignore into journalissuearticles values( S.aureus );. This study demonstrates the importance of quality control for reliable formulations based on propolis.
Keywords : A mellifera, Propolis, HPLC