Quantitative Determination of Surface Morphology of Red Blood Cell

Authors : Özlem KOCAHAN YILMAZ

Pages : 385-395

Doi:10.28979/jarnas.1206923

View : 38 | Download : 35

Publication Date : 2023-06-30

Article Type : Research Paper

Abstract :In this study, the determination of the surface morphology of red blood cell insert ignore into journalissuearticles values(RBC); from interferogram image obtained by quantitative phase imaging insert ignore into journalissuearticles values(QPI); method is presented. QPI, is an optical measurement method frequently used in recent years, allows to obtain quantitative data for different samples insert ignore into journalissuearticles values(cell, thin film surface, etc.);. Many measurement setups at the micrometer level and with nanometer precision have been designed for quantitative surface determination. Among these, white light diffraction phase microscopy insert ignore into journalissuearticles values(WDPM); is a design that combines the advantages of off-axis holography-specific speed and phase sensitivity associated with common path interferometry. Interferogram image of RBCs have been formed by the WDPM setup. Analysis of this image has been carried out by Fourier transform. As a result of this analysis, three-dimensional insert ignore into journalissuearticles values(3D);, dynamic insert ignore into journalissuearticles values(observable from all angles); and height-known profiles of RBCs have been created. From the height profiles, the parameters related to the morphology of RBCs as the projected surface area insert ignore into journalissuearticles values(PSA);, diameter insert ignore into journalissuearticles values(D);, mean corpuscular volume insert ignore into journalissuearticles values(MCV); and total surface area occupied by the cell insert ignore into journalissuearticles values(SA);, have been determined quantitatively. In addition, two-dimensional images, obtained by examining blood samples with light microscopy and scanning electron microscopy insert ignore into journalissuearticles values(SEM);, have been compared with the data achieved by WDPM. The advantages and disadvantages of WDPM and light microscopy and SEM, which are commonly used in biomedical measurements, are discussed through the results. In this way, it was possible to see the difference between QPI and traditional methods used to imaging the cell surface.
Keywords : quantitative phase imaging, interferogram, Fourier transform, light microscope, SEM