The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study

Authors : Fahri PAT, Sultan FİDAN PEDÜK, Neşe AKÇAY, Hatice Kübra KIZIL PAT, Ercan ARICAN

Pages : 719-729

Doi:10.28979/jarnas.1217912

View : 46 | Download : 39

Publication Date : 2023-09-20

Article Type : Research Paper

Abstract :Lake Beysehir, located in the Central Anatolia Region, is the biggest lake in Central Anatolia, providing drinking water and irrigation. It is Turkey\`s third-largest lake and the largest freshwater lake. Its waters are fresh water and its depth is around 8-9 m at most. The deepest point is mentioned as 12, 14, and 15 m in different sources. Although there is a lake used by such a large population in our country, there are not enough studies on microbial diversity, so revealing the diversity is important for the lake and the population around the lake. Next-generation sequencing insert ignore into journalissuearticles values(NGS); research has made it possible to sequence RNA and DNA considerably quicker and cheaply than in by assessing the PCR amplicon, we utilized NGS high-throughput methods for the metagenomic research of Lake Beyşehir sequences of 16S rDNA insert ignore into journalissuearticles values(regions V3–V4);. The analyzes revealed the phylum Proteobacteria, Verrucomicrobia, Bacteroidetes and Actinobacteria.
Keywords : Metagenomic, NGS, 16S rRNA, Ecology, Microbial diversity