IN VITRO STUDIES ON THE PROTECTIVE EFFECT OF TANNIC ACID OF U87 CELLS INDUCED BY BETA-AMYLOID

Authors : Gülüzar ÖZBOLAT, Ares ALİZADE

Pages : 213-217

Doi:10.30621/jbachs.990503

View : 25 | Download : 8

Publication Date : 2021-09-20

Article Type : Research Paper

Abstract :This study shows that tannic acid is an Alzheimer`s disease insert ignore into journalissuearticles values(AD); model cell line on amyloid-beta insert ignore into journalissuearticles values(Aβ);-induced U87 insert ignore into journalissuearticles values(human astrocytoma cell line); in β-amyloid insert ignore into journalissuearticles values(Aβ);-induced in vitro Alzheimer`s Disease insert ignore into journalissuearticles values(AD); model. We aimed to investigate the effects of antioxidants.In the study; Three groups were formed as the control group, the Aβ group, and the Aβ + tannic acid group obtained by adding tannic acid to the Aβ group. Firstly, the cytotoxic potential of TA in U87 cells was investigated by the colorimetric MTT insert ignore into journalissuearticles values(3-4,5-dimethyl-thiazolyl-2,5 diphenyltetrazolium bromide); test. To determine the antioxidant status in the cell line treated with Thymoquinone, to examine the effects of superoxide dismutase insert ignore into journalissuearticles values(SOD);, catalase insert ignore into journalissuearticles values(CAT); activities, total oxidant status insert ignore into journalissuearticles values(TOS);, and total antioxidant status insert ignore into journalissuearticles values(TAS); were measured by the ELISA method. When compared to the control group, the SOD, CAT, and TOS levels were significantly decreased in the U87 cell line exposed to Aβ; TAS levels were found to increase significantly. However, the application of tannic acid to the Aβ-U87 cell line significantly increased SOD, CAT, and TAS levels; It was found that it decreased the TOS level. In in vitro experiments, we determined that tannic acid has a protective effect by increasing antioxidant parameters in the amyloid beta-induced cell line.
Keywords : Tannic acid, Amyloid beta, U87, Alzheimer