Activation of membrane TRPM2 channel currents in primary Kv1.3-deficient mice megakaryocyte by agonist ADP-Ribose

Authors : Mustafa NAZIROĞLU

Pages : 117-121

View : 19 | Download : 10

Publication Date : 2013-08-14

Article Type : Research Paper

Abstract :Since the mechanisms that lead to melastatin-like transient receptor potential 2 insert ignore into journalissuearticles values(TRPM2); channel activation in response to ADP-Ribose insert ignore into journalissuearticles values(ADPR); are not understood, I tested the effects of ADP-Ribose on TRPM2 cation channel currents in native bone marrow megakaryocyte of mice. Kv1.3-deficient mice megakaryocyte cells were freshly isolated from bone marrow and the cells studied with the conventional whole-cell patch clamp technique. ADPR insert ignore into journalissuearticles values(1mM); was applied intracellularly through the pipette. Non-selective cation currents in the mice were consistently induced by ADPR. Current density of ADPR in the cells were significantly insert ignore into journalissuearticles values(p<0.001); higher than in control. The time courses of ADPR effects in the mice were characterized by a delay of 3.66 ± 0.69. In conclusion, TRPM2 channels were constitutively activated by intracellular ADPR currents in mice native megakaryocyte.
Keywords : TRPM2 channels, ADP Ribose, bone marrow, megakaryocyte