Effects of sub-chronic exposure of male albino rats to some insecticides on mitochondrial dysfunction and oxidative stress in the kidney with molecular docking

Authors : Mostafa TAHA, Mohamed BADAWY, Reda ABDELRAZİK, Hassan YOUNİS, Mahmoud ABOELSAAD

Pages : 0-0

Doi:10.37212/jcnos.1080039

View : 23 | Download : 9

Publication Date : 2022-04-21

Article Type : Research Paper

Abstract :The present study evaluated the adverse effects of three widely used insecticides in the Egyptian environment on mitochondrial bioenergetic and oxidative stress biomarkers in the rat kidney. Chlorpyrifos, cypermethrin, and imidacloprid were orally administrated to male albino rats at 1/50 of the LD50 for 28 days by 5 doses /week. The insecticides caused a significant in vivo decrease in the activities of mitochondrial bioenergetic biomarkers; NADH dehydrogenase, adenosine triphosphatase insert ignore into journalissuearticles values(ATPase); and mitochondrial oxidative stress biomarkers; superoxide dismutase insert ignore into journalissuearticles values(SOD);, and glutathione S- transferase insert ignore into journalissuearticles values(GST); while the malondialdehyde insert ignore into journalissuearticles values(MDA); and protein carbonyl contents insert ignore into journalissuearticles values(PCC); were significantly increased. Further, chromatography analysis demonstrated that 8-hydroxy-2`-deoxyguanosine insert ignore into journalissuearticles values(8-OH-2DG); increased considerably in rat urine as a DNA damage biomarker. The kidney deficiency was confirmed by histological examination and in silico simulation analysis insert ignore into journalissuearticles values(Molecular docking and absorption, distribution, metabolism, excretion, and toxicity insert ignore into journalissuearticles values(ADMET););. The alterations in the tested parameters were confirmed by the symptoms of histological deformation in kidney tissues, demonstrating the hazardous effects. The laboratory results showed the impact of the tested insecticides in conformity with the in silico simulation analysis insert ignore into journalissuearticles values(molecular docking and ADMET);.
Keywords : Oxidative stress, Rat kidney, Mitochondria, Insecticides, HPLC analysis, Molecular docking