Influence of Different Concentrations of Murashige and Skoog Medium on Multiple Shoot Regeneration of Staurogyne repens (Nees) Kuntze

Authors : Muhammet DOĞAN

Pages : 61-67

Doi:10.30931/jetas.1055833

View : 14 | Download : 12

Publication Date : 2022-04-30

Article Type : Research Paper

Abstract :Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental plants, medicinal and aromatic plants. Therefore, optimization of production techniques is very important. In this study, the effects of different Murashige and Skoog insert ignore into journalissuearticles values(MS); salt levels insert ignore into journalissuearticles values(25%-200%); on the in vitro production of Staurogyne repens insert ignore into journalissuearticles values(Nees); Kuntze were investigated. The shoot tip was used as explant. Different levels of MS salts significantly affected the regeneration abilities of shoot tip explants. The highest shoot regeneration rate was determined at 100% and 150% MS salt levels. The maximum regenerated shoots insert ignore into journalissuearticles values(9.13 shoots/explant); was determined in nutrient media supplemented with 150% MS salts. The minimum count of shoots insert ignore into journalissuearticles values(5.22 shoots/explant); was recorded in food media including 200% MS salts. Mean shoot lengths were between 1.65-2.27 cm. The highest length value insert ignore into journalissuearticles values(2.27 cm); was determined in the culture medium with 100% MS salts added, while the lowest length value insert ignore into journalissuearticles values(1.65 cm); was determined in the nutrient medium containing 25% MS salts. The shoots in the propagation medium were rooted in nutrient medium supplemented with 0.25 mg/L indole-3-acetic acid insert ignore into journalissuearticles values(IAA);. Then they were successfully acclimatized to external conditions.
Keywords : Culture medium, in vitro propagation, shoot regeneration, tissue culture