Effect of tocilizumab in subarachnoid hemorrhage-induced cerebral vasospasm of experimental rats

Authors : Emir İZCİ, Fatih KESKİN, Bulent KAYA, Fatma Hümryra YERLİKAYA, Gökhan CÜCE

Pages : 699-704

Doi:10.32322/jhsm.1290161

View : 50 | Download : 75

Publication Date : 2023-05-31

Article Type : Research Paper

Abstract :Aim: This study aimed to evaluate the effects of tocilizumab insert ignore into journalissuearticles values(TCZ);, a recombinant humanized, anti-human monoclonal antibody of the immunoglobulin G1k subclass, on vascular morphological changes, endothelial apoptosis, and the levels of pro-inflammatory and apoptotic cytokines, such as IL-6, tumor necrosis factor-alpha insert ignore into journalissuearticles values(TNF-α);, caspase-3, Bcl-2 associated X-protein insert ignore into journalissuearticles values(BAX);, and vascular endothelial growth factor insert ignore into journalissuearticles values(VEGF); in a rat SAH model. Material and Method: The rats were randomly assigned insert ignore into journalissuearticles values(animal study); to 4 groups KONÜDAM Experimental Animal Research Center, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, Turkey; 15/03/2019);: insert ignore into journalissuearticles values(1); normal control insert ignore into journalissuearticles values(without SAH);; insert ignore into journalissuearticles values(2); SAH insert ignore into journalissuearticles values(without treatment);; insert ignore into journalissuearticles values(3); SAH treated with saline insert ignore into journalissuearticles values(SAH + Sal.);; and insert ignore into journalissuearticles values(4); SAH treated with TCZ insert ignore into journalissuearticles values(SAH + Toc.);. The tissues were measured using enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA); kits. A series of brain and basilar artery sections were categorized into several subgroups for hematoxylin and eosin insert ignore into journalissuearticles values(H&E); staining, immunohistochemistry, and Terminal deoxynucleotidyl transferase dUTP nick end labeling insert ignore into journalissuearticles values(TUNEL); staining. Results: The levels of caspase, BAX, and IL-6 in the SAH + TOC group were significantly lower than in other groups. TCZ treatment significantly increased the lumen of the basilar artery compared with that in the SAH and SAH + SAL groups without treatment insert ignore into journalissuearticles values(p=0.002 and p=0.004 respectively);. SAH increased the apoptotic index in the endothelium compared with TCZ treatment insert ignore into journalissuearticles values(p=0.027); groups. Conclusion: It can be concluded that TCZ is safe and effective for treating experimental SAH. The results reveal clearly experimental evidence for the potential clinical application of TCZ in SAH patients.
Keywords : Subarachnoid hemorrhage, tocilizumab, vasospasm