Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq

Authors : Hasan Raheem KHUDHUR, Ghada Basil ALOMASHİ

Pages : 15-22

Doi:10.5455/jicm.30.20180422

View : 41 | Download : 9

Publication Date : 2018-06-01

Article Type : Research Paper

Abstract :Background: Cutaneous leishmaniasis insert ignore into journalissuearticles values(CL); is vector-borne disease. and endemics in most regions of Iraq especially with poor populations. ` Natural resistance-associated macrophage protein 1 insert ignore into journalissuearticles values( NRAMP1 );` gene play an essential role in susceptibility to CL and disease pathology, NRAMP1 influences a production and activation of pro-inflammatory cytokines like IL-1β and TNF-α. Pro- and anti-inflammatory cytokines play an essential role in susceptibility/resistance and the immunopathogenesis of Leishmania infection. These cytokines are crucial factors in the initiation and enhances of protective immunity against Leishmania infection. This study aimed to determine effect of polymorphism in NRAMP1 genes on cytokines secretion and their effect in susceptibility to CL infection. Materials and Methods: Samples of blood were collected from patients insert ignore into journalissuearticles values(n: 60); with CL and apparently healthy controls insert ignore into journalissuearticles values(n: 32);. Polymorphism of Nramp1 insert ignore into journalissuearticles values( D543N ); detected by PCR-RFLP technique in patients and control groups while insert ignore into journalissuearticles values(TNF-α and IL-1β); cytokine concentrations detected by ELISA technique using a quantitative sandwich enzyme immunoassay technique. Results: Results indicate to effect of Nramp1 gene polymorphism on levels of insert ignore into journalissuearticles values(IL-1β and TNF-α); cytokines and this a clearly recorded in present study were A allele is associated with lower levels of insert ignore into journalissuearticles values(TNF-α and IL-1β); in patients and control groups compression to that absorbed in allele G with statically significant insert ignore into journalissuearticles values(p ≤ 0.05);. Conclusions: Cytokines insert ignore into journalissuearticles values(IL-1β and TNF-α); plays an essential  role in the resolution of CL infection,  were its concentration  in patients serum of all age groups were significant increase in comparison to that observed in their control groups. In polymorphisms of Nramp1 insert ignore into journalissuearticles values( D543N ); gene, were A allele is associated with lower levels of insert ignore into journalissuearticles values(IL-1β and TNF-α); compression to that  absorbed in allele G, and this decreased production may be associated with susceptibility and proliferation of parasites in the macrophage.
Keywords : Cutaneous leishmaniosis, NRAMP1polymorphisms, TNF α and IL 1β, Cytokine

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