Investigation of the Relationship Between TNF-α, IL-12A, IL-12B and IFN- γ Gene Polymorphisms and Chronic Hepatitis B in Turkish Populations

Authors : Gülay BÖREKÇİ, İrem BEKALP, Özlem KANDEMİR, Ayşegül ÇETİNKAYA, Gülhan OREKİCİ TEMEL, Nurcan ARAS

Pages : 123-132

View : 18 | Download : 9

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Background/Aims: This study aimed to determine the relationship between TNF-α insert ignore into journalissuearticles values(-1031 T/C);, IL-12A insert ignore into journalissuearticles values(3`UTR G/A);, IL-12B insert ignore into journalissuearticles values(3`UTR A/C); and IFN-γ insert ignore into journalissuearticles values(+874 A/T); gene polymorphisms and chronic hepatitis B infections. Materials and Methods: 100 patients with chronic hepatitis B and 100 healthy subjects were included in this study. Approximately 2 ml of peripheral blood from patient and control groups were taken into tubes with EDTA and the genomic DNA was isolated by using the DNA isolation kit. Single nucleotide polymorphisms insert ignore into journalissuearticles values(SNPs); in TNF-α insert ignore into journalissuearticles values(-1031 T/C);, IL-12A insert ignore into journalissuearticles values(3`UTR G/A);, IL-12B insert ignore into journalissuearticles values(3`UTR A/C); and IFN-γ insert ignore into journalissuearticles values(+874 A/T); genes were investigated by using RT-PCR method. Results: It was determined that there was no statistically significant difference in terms of the allele and genotype frequencies of TNF-α insert ignore into journalissuearticles values(T/C); and IFN-γ insert ignore into journalissuearticles values(A/T); between chronic hepatitis B patients and the control group insert ignore into journalissuearticles values(p>0.05);. However, there was a statistically significant difference between the patient and control groups for IL-12A insert ignore into journalissuearticles values(G/A); and IL-12B insert ignore into journalissuearticles values(A/C); in terms of genotype and allele distributions. The frequencies of IL-12A GG and GA genotypes insert ignore into journalissuearticles values(OR: 0.814, 95% 0.369-1.795, p=0.001); in patient group, and AA genotype in control group insert ignore into journalissuearticles values(OR: 2.608 95% 1.189-5.720, p=0.001); were high. Futhermore, it was found that the frequencies of IL-12A G allele in patient group and A allele in control group insert ignore into journalissuearticles values(OR: 1.981, 95%1.115-2.462, p=0.001); were high. In terms of IL-12B genotype and allele distributions, the frequencies of AC genotype insert ignore into journalissuearticles values(OR: 0.131, 95% 0.049-0.350, p=0.001); and A allele was higher in the patient group than the control group, whereas C allele in the control group was high. insert ignore into journalissuearticles values(OR:1.657, 1.115-2.462, p=0.012);. Conclusion: Our results showed that TNF-α insert ignore into journalissuearticles values(-1031 T/C);, and IFN-γ insert ignore into journalissuearticles values(+874 A/T); polymorphism is not effective in chronic HBV infections. However, IL-12A insert ignore into journalissuearticles values(3`UTR G/A); and IL-12B insert ignore into journalissuearticles values(3`UTR A/C); polymorphisms may influence the chronicity of hepatitis B.
Keywords : Chronic hepatitis B, polymorphism, TNF α, IL 12, IFN γ, SNP