OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS

Authors : Burcu ÇAKILCI ÖZDEMİR, Bülent KURTİŞ, Gülay TÜTER, Burcu ŞENGÜVEN, Benay YILDIRIM, Gönen ÖZCAN

Pages : 1-9

Doi:10.17096/jiufd.40536

View : 26 | Download : 10

Publication Date : 2016-04-01

Article Type : Research Paper

Abstract :Purpose: Platelet-rich plasma insert ignore into journalissuearticles values(PRP); is a novel method for transferring autogenous growth factors to the wound area. The aim of this study was to evaluate the efficacy of double-application of PRP insert ignore into journalissuearticles values(DA-PRP); on bone healing in rabbit cranial defects by examining osteonectin insert ignore into journalissuearticles values(ON); and osteocalcin insert ignore into journalissuearticles values(OC); expression. Materials and Methods: Twenty-eight rabbits, each with two surgically prepared calvarial bone defects, were included in this study and divided into six groups: The defects insert ignore into journalissuearticles values(N=56); were treated with either a single-application of PRP insert ignore into journalissuearticles values(SA-PRP); insert ignore into journalissuearticles values(n=10);, a combination of SA-PRP and betatricalciumphosphate insert ignore into journalissuearticles values(SA-PRP+β-TCP); insert ignore into journalissuearticles values(n=10);, only DAPRP insert ignore into journalissuearticles values(n=8);, both DA-PRP and beta-tricalciumphosphate insert ignore into journalissuearticles values(DA-PRP+β-TCP); insert ignore into journalissuearticles values(n=8);, only beta-tricalciumphosphate insert ignore into journalissuearticles values(β-TCP); insert ignore into journalissuearticles values(n=10);, or controls insert ignore into journalissuearticles values(n=10);. The animals were sacrificed at 30th day postoperatively and samples were immunohistochemically examined for ON and OC expressions. Results: It was determined that DA-PRP did not significantly improve the ON and OC percentages achieved by SA-PRP or the controls. The three groups treated with β-TCP showed a higher percentage of ON than those treated without β-TCP insert ignore into journalissuearticles values(p<0.05);. The β-TCP treated groups and SA-PRP group demonstrated higher OC percentage than DA-PRP and control groups insert ignore into journalissuearticles values(p<0.05);. Conclusion: The present findings suggest that DAPRP did not have a significant effect on the healing of non-critical size rabbit cranial bone defects.
Keywords : Platelet Rich Plasma, osteonectin, osteocalcin, wound healing, osteogenesis