Molecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets

Authors : Vinay Kumar NADEEM FAİROZE

Pages : 65-68

Doi:10.5799/jmid.328803

View : 5 | Download : 2

Publication Date : 2016-06-01

Article Type : Research Paper

Abstract :Objective: To isolate Listeria monocytogenes from chicken and mutton meat sold at retail outlets and to characterize isolates for virulence determinants. Methods: Listeria monocytogenes was isolated from chicken and mutton meat samples using ISO: 11290 method. Multiplex PCR was performed for the detection of virulence associated genes. Results: Out of 198 retail meat samples insert ignore into journalissuearticles values(72 chicken and 126 mutton); analyzed, Listeria monocytogenes was isolated from 4.5% insert ignore into journalissuearticles values(8.3% chicken and 2.3% mutton); samples. All the 9 isolates insert ignore into journalissuearticles values(6 from chicken and 3 from mutton); belonged to 1/2a servar and carried virulence genes viz. haemolysin insert ignore into journalissuearticles values(hlyA);, phosphatidylinositol phospholipase insert ignore into journalissuearticles values(plcA);, actin polymerization protein insert ignore into journalissuearticles values(actA); and invasive associative protein p60 insert ignore into journalissuearticles values(Iap);. Conclusion: L. monocytogenes is an organism of food safety and public health significance, its recovery from the meat sold at retail outlets indicated breach in the quality assurance. J Microbiol Infect Dis 2016;6insert ignore into journalissuearticles values(2);: 65-68   
Keywords : Listeria monocytogenes, chicken, mutton, virulence marker