Pathogenic Vibrio Strains Isolated from Human Stool and Water Samples from Western Kenya

Authors : Roselida Achieng OWUOR

Pages : 1-7

Doi:10.5799/jmid.328759

View : 7 | Download : 2

Publication Date : 2016-03-01

Article Type : Research Paper

Abstract :Objective: Investigate the type of pathogenic Vibrio strains from water and stool samples collected from Migori, SonduMiriu, Nyando and Yala regions in Western Kenya. Methods: A total of 811 samples insert ignore into journalissuearticles values(596 water and 215 stool samples); were collected during the study periods of May to December 2013 and August to September 2014. Pathogenic Vibrio strains were identified through culturing in TCBS Agar, followed by oxidation, string and serological insert ignore into journalissuearticles values(polyvalent); tests, respectively. The PCR analysis was done using combined primers targeting Vibrionaceae 16SrRNA and species specific primers for V. vulnificus and V. cholerae. Results: The results showed the presence of V. vulnificus and V. cholerae. However, V. parahaemolyticus was not found in any of the samples. The PCR results for 16SrRNA, Vib 1, and Vib 2 showed polymorphism in the genes, this was an indication of cross combination of genes from more than one strain in one isolate. Conclusion: The study showed the presence of V. cholerae insert ignore into journalissuearticles values(Ogawa and Inaba); in water and human stool samples. Type B V. vulnificus was detected in the water sample collected from River Migori. This information is of essence in controlling and managing cholera in the western part of Kenya. J Microbiol Infect Dis 2016;6insert ignore into journalissuearticles values(1);: 1-7
Keywords : V vulnificus, V parahaemolyticus, PCR, V cholerae