Molecular basis of quinolone resistance in Enterobacterial isolates

Authors : Nivedita DASGUPTA

Pages : 97-102

Doi:10.5799/jmid.328959

View : 10 | Download : 4

Publication Date : 2016-09-01

Article Type : Research Paper

Abstract :Objective: Fluoroquinolones are the commonly prescribed antimicrobial for Gram-negative and Gram-positive microorganisms. The resistance against these antibiotics is of importance in therapy. The current study aimed at determination of aacinsert ignore into journalissuearticles values(6’);-Ib-cr gene within the Enterobacterial isolates and their transmission dynamics. Materials and Methods: Ninety-seven isolates of Enterobacteriaceae collected during May 2013 to October 2013 were screened by the antimicrobial susceptibility test. The plasmids from these isolates were analysed by specific Polymerase chain Reaction insert ignore into journalissuearticles values(PCR); for qnrA, qnrB, qnrD, qnrC, and aacinsert ignore into journalissuearticles values(6’);-1b-cr and the horizontal dissemination of these plasmid mediated quinolone determinants and aacinsert ignore into journalissuearticles values(6’);-1b-cr genes were determined by transformation. Results: Our analysis showed that 94 out of 97 phenotypically screened positive isolates, 85.57% insert ignore into journalissuearticles values(n = 83);, 84.53% insert ignore into journalissuearticles values(n=82); were resistant to norfloxacin, ciprofloxacin respectively. Coexisting qnrD genes was identified in 11isolates carrying aacinsert ignore into journalissuearticles values(6’);-Ib-cr gene. The PMQR and aacinsert ignore into journalissuearticles values(6’);-Ib-cr was demonstrated by transforming the plasmid carrying qnrD and aacinsert ignore into journalissuearticles values(6’);-Ib-cr gene into E. coli strain DH5ɑ. PCR assay confirmed that aacinsert ignore into journalissuearticles values(6’);-Ib-cr was present in all the transformants. Conclusion: Our report shows the presence of heterogenous resistance mechanism can lead to worrisome condition at the nosocomial level and community acquired infection. So aacinsert ignore into journalissuearticles values(6’);-Ib-cr containing strains should be promptly detected and referred to clinicians so that the treatment with non-hydrophilic FQs, such as levofloxacin and ofloxacin, or other classes of antibiotics to prevent high level resistance onset and spread. J Microbiol Infect Dis 2016;6insert ignore into journalissuearticles values(3);: 97-102 Aacinsert ignore into journalissuearticles values(6′);-1b-cr, Enterobacteriaceae, plasmid-mediated quinolone resistance, qnrD; qnrC,
Keywords : Aac 6′, 1b cr, Enterobacteriaceae, plasmid mediated quinolone resistance, qnrD, qnrC