Non-typhoidal Salmonella isolates from livestock and food samples, Kolkata, India

Authors : Sudhanthiramani SUDHANTHİRAKODİ

Pages : 113-120

Doi:10.5799/jmid.vi.328974

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Publication Date : 2016-09-01

Article Type : Research Paper

Abstract :Objective: Non-typhoidal Salmonella insert ignore into journalissuearticles values(NTS); is a major foodborne pathogen in both developed and developing countries. The study was undertaken to determine the prevalence, serotype distribution and antimicrobial resistance profiles of NTS isolates from livestock and food samples collected in and around Kolkata during 2012-2013. Materials and methods: A total of 500 samples insert ignore into journalissuearticles values(animal, poultry and raw food); from 12 different places randomly collected in and around Kolkata were processed as per standard procedures and analyzed for NTS. Serotyping of the isolates was done followed by determination of antimicrobial resistance profiles and Minimum inhibitory concentrations following disc diffusion and E-test methods. Results: A total of 53 insert ignore into journalissuearticles values(10.6%); NTS isolates were recovered, with highest isolation from chicken insert ignore into journalissuearticles values(40%);, followed by beef and poultry cloacal samples insert ignore into journalissuearticles values(22% each);. Total 11 Salmonella serovars were identified with predominance of Salmonella enterica serovar Typhimurium insert ignore into journalissuearticles values(S. Typhimurium); insert ignore into journalissuearticles values(30.2%); followed by S. Kentucky insert ignore into journalissuearticles values(22.6%);, S. Idikan insert ignore into journalissuearticles values(15.1%); and other serovars. This is the first report of isolation of S. Idikan from India. Thirty-two insert ignore into journalissuearticles values(60.4%); isolates were pan susceptible to all drugs tested. Percentage distribution of resistant NTS against antimicrobials were: nalidixic acid insert ignore into journalissuearticles values(24.5%);, ampicillin insert ignore into journalissuearticles values(17%);, amoxicillin insert ignore into journalissuearticles values(17%);, streptomycin insert ignore into journalissuearticles values(15.1%);, tetracycline insert ignore into journalissuearticles values(11.3%);, chloramphenicol insert ignore into journalissuearticles values(9.4%);, co-trimoxazole insert ignore into journalissuearticles values(9.4%); and ciprofloxacin insert ignore into journalissuearticles values(5.7%);. Only one isolate insert ignore into journalissuearticles values(S. Senftenberg); was resistant to both fluroquinolones and third generation cephalosporins. Multidrug resistance insert ignore into journalissuearticles values(≥3 classes of antimicrobials); was observed in 13.2% NTS isolates. Conclusion: High prevalence of NTS in poultry and meat samples in Kolkata poses a potential risk of foodborne disease in humans. J Microbiol Infect Dis 2016;6insert ignore into journalissuearticles values(3);: 113-120
Keywords : Non typhoidal Salmonella, antimicrobial resistance, animals, poultry, food samples