Mycological and Serological Study of Invasive Aspergillosis in A Tertiary Care Hospital

Authors : Sumanpreet KAUR, Veenu GUPTA, Deepinder Kaur CHHİNA, Akashdeep SİNGH, Daaman SHARMA

Pages : 8-12

Doi:10.5799/jmid.394584

View : 31 | Download : 6

Publication Date : 2018-03-15

Article Type : Research Paper

Abstract :Objectives: Invasive aspergillosis insert ignore into journalissuearticles values(IA); has emerged as a major life threatening infection in high risk patients. Recent advances have led to early diagnosis of IA by detecting Aspergillus antigens in serum particularly Galactomannan insert ignore into journalissuearticles values(GM);. Objective of the study was to correlate fungal smear, culture and Galactomannan assay in patients with suspected IA and to categorize them into “proven,” “probable,” and “possible,” cases as per European Organization for Research and Treatment of Cancer and Mycoses study group case definitions insert ignore into journalissuearticles values(EORTC/MSG); criteria. Methods: The prospective study was conducted over a period of one year in the department of Microbiology, Dayanand Medical College and Hospital, Ludhiana. A total of 319 patients with suspicion of IA were included in the study. GM antigen detection test was done from serum samples. KOH mount was done and samples were inoculated on Sabouraud’s dextrose agar insert ignore into journalissuearticles values(SDA);. Growth obtained was analyzed as per standard protocol. Results: GM assay positivity among suspected patients of IA was 47%. The mean age group affected was 51.8 years insert ignore into journalissuearticles values(51.8±15.2); and majority was males insert ignore into journalissuearticles values(78.1%);. The patients were categorized as Proven IA insert ignore into journalissuearticles values(0%);, Probable IA insert ignore into journalissuearticles values(52.7%);, Possible IA insert ignore into journalissuearticles values(41.4%); and No IA insert ignore into journalissuearticles values(5.9%);. Aspergillus flavus was the most common isolate insert ignore into journalissuearticles values(77.4%); Conclusion: The study demonstrated that serological tests have an edge over routine smear and culture for the diagnosis of invasive aspergillosis. Thus, Galactomannan assay is a useful diagnostic for early detection of IA in high risk patients. J Microbiol Infect Dis 2018; 8insert ignore into journalissuearticles values(1);:8-12
Keywords : Galactomannan assay, Invasive aspergillosis, Fungal culture, Fungal smear

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