Role of Phenotypic Switching in Stability and Persistence of Pseudomonas aeruginosa Biofilms

Authors : Zulfiqar Ali MİRANİ, Shaista UROOJ, Fouzia ZEESAHN, Muhammad Naseem KHAN, Mubashir AZİZ, Izhar Ahmed SHAİKH, Abdul Basit KHAN

Pages : 10-17

Doi:10.5799/jmid.700505

View : 15 | Download : 8

Publication Date : 2020-03-15

Article Type : Research Paper

Abstract :Objectives: Objective: This study was designed to explore the role of different phenotypes of P. aeruginosa in the development, stability and persistence of biofilm. Methods: A total of seventeen insert ignore into journalissuearticles values(17); waterborne biofilm producing strains of P. aeruginosa were studied. These isolates were identified on the basis of typical phenotypic characters and the tube method was used for the study of biofilms. Population and phenotypic variance were studied by the drop plate method. The hydrophobicity of strains was evaluated by the bacterial adhesion to apolar solvent test. Results: Study showed that the subject isolates of P. aeruginosa adopted a biofilm life style after 36 h of incubation at 35 °C. After 24 h the adhesion started, but it was reversible and easily dispersed by simple washing. However, after 36 h the irreversible adhesion was noticed. The biofilm consortia harbor three different phenotypes: i. wild types, showed typical P. aeruginosa characters on Cetrimide agar; ii. Slow growers, showed poor pigmentation and take >36 h for colony development, and iii. Small colony variants insert ignore into journalissuearticles values(SCVs); are metabolically inactive and producing pinpointed non pigmented colonies. Comparative analysis showed that these phenotypes i.e. SCVs were highly hydrophobic and persistent in biofilm consortia due to the production of excessive amounts of exopolysaccharides. Conclusions: This study showed that phenotypic heterogeneity is a characteristic feature of P. aeruginosa biofilms and all of these phenotypes have a major role in stability and persistence of biofilm consortia. J Microbiol Infect Dis 2020; 10insert ignore into journalissuearticles values(1);:10-17.
Keywords : Biofilms, Hydrophobicity, P aeruginosa, Phenotypes, SCVs