Genetic Profiling of Methicillin-resistant Staphylococcus aureus in An African Hospital by Multiplex-PCR

Authors : Shuaibu Suleiman ADEİZA, Josiah Ademola ONAOLAPO, Busayo Olalekan OLAYINKA

Pages : 144-153

Doi:10.5799/jmid.790286

View : 9 | Download : 5

Publication Date : 2020-09-15

Article Type : Research Paper

Abstract :Objectives: Methicillin resistant Staphylococcus aureus insert ignore into journalissuearticles values(MRSA); nasal carriage often precedes infection. This work aims to genetically profile the MRSA carriage of resistant genes insert ignore into journalissuearticles values(mecA, blaZ and tetM); and certain virulence genes insert ignore into journalissuearticles values(nuc, pvl, spa and scn); obtained from human nasal cavity. Methods: Phenotypically confirmed Staphylococcus aureus were Screened for oxacillin resistance and assessed for β-lactamase production. Further confirmation of identity obtained by multiplex PCR of the 16SrRNA, mecA, tetM, pvl, nuc, spa and scn genes. DNA gel fingerprint analysis was carried out and the bands were clustered in a dendrogram. Results: The study analysed 81 Staphylococcus aureus isolates for Methicillin resistance out of which 38 insert ignore into journalissuearticles values(46.9%); were MRSA. The DNA extraction method was effective for all MRSA isolates evaluated. All phenotypic MRSA isolates insert ignore into journalissuearticles values(100%); were positive for the S. aureus specific 16SrRNA gene confirming that these isolates were S. aureus strains. The nuc gene was detected in 19/38 insert ignore into journalissuearticles values(50%); of the MRSA isolates, blaZ gene in 14/38 insert ignore into journalissuearticles values(36.8%); and tetM in 18/38 insert ignore into journalissuearticles values(47.4%);. Conclusions: The need for improved vigilance to recognize MRSA strains cannot be overemphasized. If MRSA control is not tracked, these strains will increase exponentially. J Microbiol Infect Dis 2020; 10insert ignore into journalissuearticles values(3);:144-153.
Keywords : Methicillin resistant Staphylococcus aureus, PCR simulation, mecA, Electrophoretogram