Evaluation of Rifampicin-resistant Tuberculosis in Pediatric Patients by GeneXpert MTB/RIF

Authors : Ashish WİLLİAM, Yogita RAI, Ravinder KAUR

Pages : 81-87

Doi:10.5799/jmid.951506

View : 32 | Download : 3

Publication Date : 2021-06-15

Article Type : Research Paper

Abstract :Objectives: India has the largest burden of MDR-TB worldwide, with an annual incidence of 1,30 000 patients. Multidrug-resistant tuberculosis insert ignore into journalissuearticles values(MDR-TB); carries a poor prognosis, a high mortality rate, and treatment success rates as low as 65%. The mortality in India is estimated to be about 480,000 per year. The aim of the study was to evaluate Rifampicin-resistant Tuberculosis and its distribution by GeneXpert in a tertiary care hospital. Methods: A total of 2864 samples were processed from the patients attending outpatient departments and indoor wards as per the pediatrician`s request. Acid-fast bacilli insert ignore into journalissuearticles values(AFB); smear microscopy was done on all samples by Acid-fast staining for early diagnosis followed by GeneXpert MTB/RIF insert ignore into journalissuearticles values(CBNAAT); testing. Specimens were transported and stored at 2–8 °C prior to processing for CBNAAT. Results were read and reported within 2 hours. Results: A total of 2864 samples were tested for TB using CBNAAT insert ignore into journalissuearticles values(including 645 [22.5%] extra-pulmonary and 2219 [77.5%] pulmonary samples);. The test results were positive in a total of 346 insert ignore into journalissuearticles values(12%); samples by CBNAAT. The positivity is highest in the age group >10 years and in sputum samples insert ignore into journalissuearticles values(37.28%); followed by pus samples insert ignore into journalissuearticles values(23.26%); in pulmonary and extrapulmonary distribution. Out ZN staining was positive in 244 insert ignore into journalissuearticles values(8.5%); specimens. There were 102 insert ignore into journalissuearticles values(29.5%); CBNAAT positive specimens which showed negative results for Acid-fast bacilli insert ignore into journalissuearticles values(70.52% Sensitivity);. Among 346 TB-positive patients, 10.46% samples were pulmonary and 17.67% were extrapulmonary. It was observed that in positive CBNAAT patients, the prevalence of Rifampicin resistance was 12.72% i.e. 44 samples which include 31insert ignore into journalissuearticles values(13.36%); and 13insert ignore into journalissuearticles values(11.40%); pulmonary and extra-pulmonary samples respectively. Among the Rifampicin resistant samples, there were 29.55% samples that were extrapulmonary. Conclusion: GeneXpert MTB/RIF is a very rapid diagnostic assay that provides information regarding the mutation pattern of RIF resistance in MTB isolates. J Microbiol Infect Dis 2021; 11insert ignore into journalissuearticles values(2);:81-87.
Keywords : CBNAAT, Tuberculosis, GeneXpert MTB RIF

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