Efficiency of Real-Time PCR in the Diagnosis of Community-Acquired Bacterial Meningitis in Children

Authors : Shahid RAZA, Bimal DAS, Rama CHAUDHRY, Vinay GOYAL, Rakesh LODHA, Seema SOOD, Hitender GAUTAM, Arti KAPİL

Pages : 47-53

Doi:10.5799/jmid.1130082

View : 5 | Download : 5

Publication Date : 2022-06-15

Article Type : Research Paper

Abstract :Objectives: Community-acquired bacterial meningitis insert ignore into journalissuearticles values(CABM); is a life-threatening condition and remains a public health concern despite various efforts to prevent it. This study aimed to detect the bacteria causing CABM in children by Real-Time PCR. Methods: In total, 178 Cerebrospinal fluid insert ignore into journalissuearticles values(CSF); samples from suspected meningitis cases were collected and subjected to cell count, biochemical, microbiological, and molecular analysis. Bacteria grown on blood and chocolate agar were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry insert ignore into journalissuearticles values(MALDI-TOF MS);. DNA from CSF was extracted and used to detect bacteria by Real-Time PCR using TaqMan Probe. Results: Fifty insert ignore into journalissuearticles values(28.09%); patients were diagnosed with confirmed meningitis. Of them, 46 insert ignore into journalissuearticles values(25.84%); were Real-Time PCR, and four insert ignore into journalissuearticles values(2.25%); were culture and Real-Time PCR positive. Out of 50 bacteria detected, S. pneumoniae insert ignore into journalissuearticles values(n=35, 19.7%); was the leading causative bacteria and was followed by H. influenzae insert ignore into journalissuearticles values(seven, 3.93%);, E. coli insert ignore into journalissuearticles values(five, 2.80%);, S. agalactiae insert ignore into journalissuearticles values(two, 1.12%);, and N. meningitidis insert ignore into journalissuearticles values(one, 0.56%);. Most of the S. pneumoniae insert ignore into journalissuearticles values(18 isolates, 51.4%); were isolated from 3-24 months of children, and in neonates, E. coli was the predominant bacteria. When CSF culture was the gold standard for diagnosis, the sensitivity and specificity of Real-Time PCR for S. pneumoniae were 100% insert ignore into journalissuearticles values(95%CI: 15.81-100%); and 81.25% insert ignore into journalissuearticles values(95%CI: 74.69-86.73%);, respectively. Conclusion: Streptococcus pneumoniae remains the leading organism of CABM in children despite vaccination and advancement in diagnosis. Real-time PCR has emerged as a vibrant diagnostic molecular appliance. Hence, Regular surveillance is crucial to curb the burdens and trends of CABM in children.
Keywords : Streptococcus pneumoniae, Community acquired bacterial meningitis, CSF, Real Time PCR