Evaluation of Total Polyphenol Content, Antioxidant Activity and Chemical Composition of Methanolic Extract from Allium Kharputense Freyn et. Sint. and Determination of Mineral and Trace Elements

Authors : Erdal Yabalak, Ahmet Murat Gizir

Pages : 691-708

Doi:10.18596/jotcsa.315094

View : 29 | Download : 9

Publication Date : 2017-07-01

Article Type : Research Paper

Abstract :Allium kharputense Freyn Et. Sint. insert ignore into journalissuearticles values( A. kharputense ); was analyzed for total polyphenol content, antioxidant activity, chemical composition and mineral and trace element composition. Total polyphenol content was determined as 257 mg GA 100 g -1 dried weight by using Folin-Ciocalteu method. DPPH test was performed for antioxidant activity analysis. IC 50 values of gallic acid and Trolox were determined as 0.02642 mg mL -1 and 0.225 mg mL -1 , respectively. IC 50 value of A. kharputense was found as 2.186 mg mL -1 . DPPH free radical-scavenging activity of extract from 1 mg of A. kharputense was determined 0.01207 mg GAE and 0.1029 mg TrE, respectively. GC-MS was used to determine chemical composition. Dimethyl trisulfide, methyl insert ignore into journalissuearticles values(methylthio);methyl disulfide, 2-Pyrrolidinone, 4-vinylphenol, 2-Phenylpyrrole , norharmane , linoleic acid, palmitic acid, γ-Tocopherol and α-Tocopherol are some of 40 compounds which were detected in methanolic extracts of A. Kharputense. 20 Mineral and trace elements were determined along with their levels by ICP-MS using microwave digestion procedure for preparing acid extracts.
Keywords : Antioxidant activity, Allium Kharputense Freyn Et Sint, Polyphenolic content, DPPH

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