Optimization of Ultrasound-Assisted Extraction of Antioxidants from Apium graveolens L. Seeds using Response Surface Methodology

Authors : Şule DİNÇ ZOR, Merve BAT, Ayşegül Peksel, Güzin ALPDOĞAN

Pages : 915-930

Doi:10.18596/jotcsa.324848

View : 46 | Download : 8

Publication Date : 2017-09-11

Article Type : Research Paper

Abstract :In this study, optimum conditions for ultrasound-assisted extraction insert ignore into journalissuearticles values(UAE); of antioxidants from Apium graveolens   L. seeds were investigated by Response Surface Methodology insert ignore into journalissuearticles values(RSM);. A Box-Behnken Design insert ignore into journalissuearticles values(BBD); was used to evaluate the effect of sonication time insert ignore into journalissuearticles values(5, 10, 15 min);, ultrasound power insert ignore into journalissuearticles values(60, 120, 180 W); and the ratio of extraction solvent in terms of methanol insert ignore into journalissuearticles values(0, 50, 100%); on antioxidant capacity. The optimal UAE conditions for the parameters investigated were 11 min of sonication time, ultrasound power of 131 W and 100% methanol as an extraction solvent. Under these conditions, UAE of antioxidants from the seeds achieved a maximum of 95.08% in respect to 1,1-diphenyl-2-picryl hydrazyl insert ignore into journalissuearticles values(DPPH); radical scavenging activity. Additionally, the high value of the adjusted coefficient of determination insert ignore into journalissuearticles values( R 2 adj = 0.9192); and the non-significant difference between experimental and predicted values confirmed the validity of the quadratic polynomial model. Hence, UAE is a suitable, fast, economical and practical technique for the extraction of antioxidants from Apium graveolens  L. seeds.
Keywords : Apium graviolens L seed, ultrasound assisted extraction, optimization

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