Application of Oxidative Coupling Reaction using Brucine and Sodium Periodate as Chromogenic Reagent for the Assay of Perindopril Erbumine in Formulations

Authors : Thuttagunta MANIKYA SASTRY, Ramakrıshna KARIPEDDI

Pages : 803-810

Doi:10.18596/jotcsa.757440

View : 19 | Download : 12

Publication Date : 2021-08-31

Article Type : Research Paper

Abstract :The drug Perindopril Erbumine insert ignore into journalissuearticles values(PE);, an ACE inhibitor, and can be used to treat the patients with hypertension and cardiac failure problems. A sensitive, inexpensive, and precise analytical technique has been developed for the estimation of Perindopril in bulk and formulations. The procedure involves the development of color by forming an oxidative coupling reaction between drug insert ignore into journalissuearticles values(PE); and Brucine/IO4-);. The formed colored species were measured at max=520 nm. The developed method showed linearity within the concentration limits of 8-24 μg mL-1. The linear correlation coefficient insert ignore into journalissuearticles values(r); and molar absorptivity were found to be 0.9999 and 9.16 x 103 mol-1.cm-1. % Recovery ± SD values were in the range of 99.16 - 100.7 insert ignore into journalissuearticles values(± 0.41 - ± 0.8); insert ignore into journalissuearticles values(n=3); which indicates the accuracy of the developed method. The interference of other excipients that are commonly present in formulations is found to be negligible. Precision and accuracy of the proposed method were confirmed by Student’s t-test and F-tests at 95% confidence limits with insert ignore into journalissuearticles values(n-1); degrees of freedom. The validity parameters of the proposed method were calculated by ICH guidelines.
Keywords : Spectrophotometry, Perindopril Erbumine, Brucine, coversyl and perigard DF