Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity

Authors : Merve BAT ÖZMATARA, Fatma ERTAN

Pages : 29-36

Doi:10.18596/jotcsa.952065

View : 9 | Download : 8

Publication Date : 2022-02-28

Article Type : Research Paper

Abstract :Laccase is one of the enzymes that catalyze the oxidation of phenolic and non-phenolic substrates and show encouraging potential as a biocatalyst in the synthesis of bioactive compounds. It is known that phenolic acids have an antioxidant effect. Bovine serum albumin insert ignore into journalissuearticles values(BSA); shows gelling activity, and nutraceutical binding ability but it does not show antioxidant activity. In this study, BSA which has no antioxidant activity using laccase, started to show antioxidant activity with gallic acid insert ignore into journalissuearticles values(GA); conjugation. The synthesized conjugates were analyzed by polyacrylamide gel electrophoresis insert ignore into journalissuearticles values(PAGE);, ultraviolet–visible spectrophotometry insert ignore into journalissuearticles values(UV-Vis);, and Fourier-transform infrared spectroscopy insert ignore into journalissuearticles values(FTIR);. Radical scavenging capacity for antioxidant activity was measured. GA-functionalized-BSA displayed greatly improved 2,2`-azinobis-insert ignore into journalissuearticles values(3- ethylbenzothiazoline-6-sulfonic acid); insert ignore into journalissuearticles values(ABTS); and 1,1-diphenyl-2- picryl-hydrazyl insert ignore into journalissuearticles values(DPPH); radical scavenging capacities, compared with the untreated BSA. Protein–flavonoid conjugates can improve the natural properties of proteins, being promising products to be used in medical, food and polymer fields where antioxidant ability is an essential feature.
Keywords : Laccase, bioconjugate, bsa, gallic acid, antioxidant activity