EPR-based study to monitor Free Radicals in Treated Silk Fibroin with Anthocyanins

Authors : Vafa ATAYEVA, Rasim ASLANOV

Pages : 1055-1062

Doi:10.18596/jotcsa.1011273

View : 17 | Download : 6

Publication Date : 2022-11-30

Article Type : Research Paper

Abstract :Bioactive materials of natural origin have great demand in industry and medicine due to their versatility and useful properties. The main purpose of this work is to prepare biocomposites for the dual purpose of modified silk fibroin insert ignore into journalissuearticles values(Bombyx mori L.);, which protects against the destructive effects of bioactive, antioxidant and ultraviolet rays. For this purpose, an aqueous extract of autumn leaves of the anthocyanin-rich smoke tree plant insert ignore into journalissuearticles values(Cotinus coggygria L.); was applied. 2% thiourea solution was used to increase the durability of the modified SF to external influences and for use in textiles. The intensity of free radicals in silk fibroin-anthocyanin insert ignore into journalissuearticles values(SFA); and silk fibroin-anthocyanin-thiourea insert ignore into journalissuearticles values(SFAT); biocomposites modified by the Electron Paramagnetic Resonance insert ignore into journalissuearticles values(EPR); method was studied. Maximum adsorption time was determined 20 minutes and the intensity of free radicals in SFA bio-composite was 80-85% and in SFAT biocomposite 50-55% in relation to silk fibroin untreated. For biomedical use of SFA, the radical scavenger activity kinetics were studied on a UV-2700 spectrophotometer and radical capture activity was calculated: RSA% insert ignore into journalissuearticles values(bioextract); = 73.52 ± 0.5, RSA% insert ignore into journalissuearticles values(SF); = 6.42 ± 0.4, RSA% insert ignore into journalissuearticles values(SFA); = 45.23 ± 0.8
Keywords : EPR spectroscopy, free radical, silk fibroin, autumn leaves, anthocyanins, antioxidant