DEVELOPMENT OF A GOLD NANOPARTICLE BASED ELECTROCHEMICAL BIOSENSOR FOR DETECTION OF PHENOLIC COMPOUNDS

Authors : İlker Polatoglu, Melike Kızılkaya, Ülfet Eren

Pages : 115-126

View : 13 | Download : 11

Publication Date : 2017-02-23

Article Type : Other Papers

Abstract :In this study, an enzymatic biosensor was developed with gold nanoparticles and its performance was tested for detection of phenolic compounds. Glassy carbon working electrode insert ignore into journalissuearticles values(GCE); was covered with different combinations of chitosan insert ignore into journalissuearticles values(Chit); as a support, gold nanoparticle insert ignore into journalissuearticles values(GNP); and tyrosinase enzyme insert ignore into journalissuearticles values(T); to form the enzymatic biosensor. The sensor components insert ignore into journalissuearticles values(Chit, GNP and T); were characterized by cyclic voltametry insert ignore into journalissuearticles values(CV); and electrochemical impedance spectroscopy insert ignore into journalissuearticles values(EIS); while its performance was tested by chronoamperometry method for catechol insert ignore into journalissuearticles values(as a model phenolic compound); selectivity. Increase in sensor signal was observed depending on high conductivity of gold nanoparticles. The developed sensor has wide linear range insert ignore into journalissuearticles values(0.046-50 µM);, low detection limit insert ignore into journalissuearticles values(13.8 nM); and high sensitivity insert ignore into journalissuearticles values(1.144 A/M);. The results indicate that this kind of biosensors is potential candidates for cheap, fast and simple detection of phenolic compounds. 
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