Effect of GLYMO/TEOS/MTEOS Sol-Gel Film Thickness on the Responses of Glucose Biosensor

Authors : Nizamettin DEMİRKIRAN, Ergun EKİNCİ

Pages : 17-24

View : 20 | Download : 9

Publication Date : 2017-10-20

Article Type : Research Paper

Abstract :800x600 Normal 0 21 false false false TR X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:`Table Normal`; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:``; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:`Calibri`,sans-serif; mso-bidi-font-family:`Times New Roman`;} In the present study, the effect of the film thickness on the responses of a glucose biosensor was investigated. The modified platinum electrodes used in the study were constructed by immobilization of glucose oxidase under a film layer of the sol-gel coating solution. The sol-gel coating solutions were prepared by using insert ignore into journalissuearticles values(3-Glycidoxylpropyl); trimethoxysilane insert ignore into journalissuearticles values(GLYMO);, tetraethoxysilane insert ignore into journalissuearticles values(TEOS);, and methyltriethoxysilane insert ignore into journalissuearticles values(MTEOS);. Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide released by the enzymatic reaction between glucose and glucose oxidase. It was found that the amperometric responses of the sensor decreased with increasing the volume of coating solution dropped on the adsorbed enzyme. It was determined that the film thickness has a significantly influence on the sensor responses. It was observed that the amperometric responses decreased by 85% when the volume of the coating solution dropped on the electrode increased from 4 µL to 12 µL .
Keywords : Biosensor, glucose, glucose oxidase, sol gel