Comparison of the Efficacy of Enrofloxacin and Lactobacillus Plantarum Cell-Free Supernatant Treatments on Vaginitis in Ewes

Authors : Barış GÜNER, İhsan KISADERE, Hakan TAVŞANLI, Serpil KAHYA DEMİRBİLEK, Abdulkadir KESKİN

Pages : 105-111

Doi:10.30782/jrvm.1139354

View : 21 | Download : 9

Publication Date : 2022-12-31

Article Type : Research Paper

Abstract :The aim of the study was to investigate the effect of different intravaginal treatment strategies on the vaginal discharge score, vaginal microbiota, bacterial and Enterobacteriaceae counts in nulliparous Merino ewes. All ewes insert ignore into journalissuearticles values(n=45); received intravaginal sponges containing 60 mg medroxyprogesterone acetate for 7 days and allocated into three equal groups insert ignore into journalissuearticles values(n=15);. Sponges were injected Lactobacillus plantarum cell-free supernatant insert ignore into journalissuearticles values(SUPER);, enrofloxacin insert ignore into journalissuearticles values(ENRO);, or physiologic saline insert ignore into journalissuearticles values(CON); prior to sponge insertion. At sponge removal, 500 IU equine chorionic gonadotropin insert ignore into journalissuearticles values(eCG); were administered in all ewes. For the detection of vaginal microbiota, bacterial and Enterobactericeae counts, samples were collected prior to sponge insertion, at sponge withdrawal, and 48 h later after sponge withdrawal. Vaginal discharge score was not different in ENRO insert ignore into journalissuearticles values(2.26±0.18); and SUPER insert ignore into journalissuearticles values(2.20±0.14); compared to CON insert ignore into journalissuearticles values(2.46±0.16);. The time-dependent alteration was significant for the mean bacterial and Enterobacteriaceae count in all groups insert ignore into journalissuearticles values(P<0.05);. Bacterial counts were found to be lower in ENRO insert ignore into journalissuearticles values(5.50±0.17); than SUPER insert ignore into journalissuearticles values(6.31±0.19); and CON insert ignore into journalissuearticles values(6.07±0.15); at sponge removal insert ignore into journalissuearticles values(P<0.05);. In addition, SUPER insert ignore into journalissuearticles values(3.74±0.21); and ENRO insert ignore into journalissuearticles values(3.49±0.27); had lower Enterobacteriaceae counts compared to CON insert ignore into journalissuearticles values(4.78±0.21); at sponge removal insert ignore into journalissuearticles values(P<0.01);. The most frequently isolated bacteria species were Trueperella pyogenes insert ignore into journalissuearticles values(28.9%); and Escherichia coli insert ignore into journalissuearticles values(46.7%);. In conclusion, treatment with enrofloxacin or cell-free supernatant decreased the Enterobacteriaceae counts in ewes. Comprehensive studies are needed to assess the effectiveness of lactic acid bacteria as an antibiotic-free treatment strategy on vaginitis in ewes that were synchronized with progesterone impregnated intravaginal sponge.
Keywords : Antibiotic, Bacterial count, Lactobacillus plantarum, Sheep, Vaginitis