INVESTIGATION OF RECENTLY ABUSED DRUGS IN DRIED BLOOD SPOTS USING FTA CARDS BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY: VALIDATION AND APPLICATION TO REAL SAMPLES

Authors : Yeter EROL ÖZTÜRK

Pages : 251-263

Doi:10.59313/jsr-a.1342292

View : 68 | Download : 23

Publication Date : 2023-09-30

Article Type : Research Paper

Abstract :A robust and selective liquid chromatography-tandem mass spectrometry insert ignore into journalissuearticles values(LC-MS/MS); method was established for the analysis of 19 illicit drugs and metabolites in whole blood dried blood spots insert ignore into journalissuearticles values(DBS); using FTA cards. Samples prepared using Ahlstrom Munksjö Gensaver™ Colorcards were extracted with methanol. Analytical separation of the analytes was maintained using an Agilent Poroshell column with mobile phase A insert ignore into journalissuearticles values(0.1% formic acid in water); and B insert ignore into journalissuearticles values(methanol);. Multiple Reaction Monitoring insert ignore into journalissuearticles values(MRM); with positive ionisation on LC-MS/MS was used to develop the method. The method was validated and met the acceptance criteria with acceptable results for carry-over, linearity, specificity, sensitivity, accuracy, precision, matrix effect and recovery. The method was applied to positively reported whole blood samples from patients suspected of drug abuse. Good quantitative agreement was obtained between the DBS and whole blood methods. Application to real DBS samples showed that this method is a good alternative and useful technique for the detection of drugs of abuse in forensic toxicology and appears to provide a good alternative storage condition.
Keywords : DBS, LC MS MS, drug abuse, real samples, illicit drugs

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