Paper-Based Biosensor System for Fast and Sensitive Phenolic Compounds Detection

Authors : Nımet YILDIRIM TİRGİL

Pages : 13-21

Doi:10.34088/kojose.887169

View : 22 | Download : 5

Publication Date : 2022-05-31

Article Type : Research Paper

Abstract :In this original paper, the development of a paper-based, sensitive, low-cost, quantitative and consistent biosensor system was demonstrated for the suitable “naked-eye” detection of phenolic compounds insert ignore into journalissuearticles values(specifical catechol);. The Paper-based system depends on the enzymatic color-changing reaction that observed by using chromogenic agent of 4-AAP insert ignore into journalissuearticles values(4-aminoantipyrine); which has a specific color changing reaction in the presence of horseradish peroxidase insert ignore into journalissuearticles values(HRP); enzyme, phenol and H2O2. The visual result was associated with the catechol concentration by using image processing software to evaluate the quantitative detection. The developed biosensor system demonstrated a linear detection range from catechol between 2,5 µM to 100 µM with 2.6% to 9.3 % of sd results. The detection limit was also calculated as 2,25 µM. Specificity, selectivity, stability and direct real sample analysis demonstrated the potential applicability of the developed paper-based biosensor system for catechol detection in food samples with minimal investment and an easy-to-use method.
Keywords : Biosensors, Food quality, Paper based sensor, Phenol detection