Identification and Prevalence of Ralstonia Solanacearum from Potato Fields of Kyrgyzstan

Authors : Tinatin Döölotkeldieva, Saykal Bobuşeva

Pages : 1-9

View : 16 | Download : 15

Publication Date : 2014-06-01

Article Type : Research Paper

Abstract :For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt insert ignore into journalissuearticles values(quarantine for the country object); was obtained and identified by enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA); and biochemical methods. Three potato insert ignore into journalissuearticles values(Solanum tuberosum); cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed during storage. For ELISA was used Ralstonia solanacerium PathoScreen R Kit DAS ELISA insert ignore into journalissuearticles values(Agdia product, USA);.Isolated races of Ralstonia solanacearum by biochemical characteristics were classified as a 3-biotype.
Keywords : the potato Solanum tuberosum, cultivars, identification of Ralstonia solanacearum, ELISA assay, biochemical tests