Determination of Amino Acid Composition of Cow’s Milk by Liquid Chromatography Using Precolumn Derivatization

Authors : A MAZHİTOVA, A KULMYRZAEV

Pages : 25-34

View : 14 | Download : 9

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :The application of the procedure of pre-column derivatization with diethyl ethoxymethylenemalonate in the determination of the amino acid content of milk by reversedphase chromatography with diode-matrix detection was investigated. The influence of the pH on the response factor was established. The conditions of modification of amino acids during sample preparation procedure were optimized. A complete separation of 18 amino acids was achieved. The most abundant amino acid in cow`s milk was glutamic acid, the concentration of which twice that of leucine and lysine, and three times greater than aspartic acid
Keywords : Аминокислота, белки, молоко, хроматография