Novel CA-125 antigen determination in serum by electrochemical methods with onion oil-containing organo-hydrogels

Authors : Ömer ER, Duygu ALPASLAN, Tuba ERŞEN DUDU, Hilal DEMİR KIVRAK

Pages : 124-135

Doi:10.51354/mjen.1242313

View : 23 | Download : 24

Publication Date : 2023-07-01

Article Type : Research Paper

Abstract :CA-125 antigen is a glycoprotein that can be found at distinct levels in blood samples according to the phases of ovarian cancer. Herein, we designed novel onion oil-organo-hydrogels insert ignore into journalissuearticles values(OOHGs); to detect CA-125 antigen at high sensitivity and selectively via electrochemical methods. OOHGs produced are characterized by swelling analysis and Fourier Transform Infrared Spectroscopy insert ignore into journalissuearticles values(FT-IR);. Cyclic voltammetry insert ignore into journalissuearticles values(CV);, Electro impedance spectroscopy insert ignore into journalissuearticles values(EIS);, and Differential pulse voltammetry insert ignore into journalissuearticles values(DPV); techniques in the potentiostat triple electron system are used for performing the electrochemical measurements. Performances and electron transfer resistances of OOHGs and OOHG+CA-125s are researched via CV and EIS, and the sensitivity properties such as LOD and LOQ of the sensor are determined via DPV. OOHG-2 among OOHGs produced exhibited the highest performance with 0.8151 mA/cm2 insert ignore into journalissuearticles values(815.1 A/cm2); value at determining CA-125 in serum medium. Moreover, this electrode is found that exhibit a wide linear range like a 1-500 ng/mL concentration range. The limit of quantification insert ignore into journalissuearticles values(LOQ); and the lowest of detection insert ignore into journalissuearticles values(LOD); for the OOHG-2 electrode are calculated as 0.531 U/mL and 0.265 U/mL insert ignore into journalissuearticles values(S/N=3);, respectively. Further, the CA-125 antigen of the OOHG-2 electrode in interference results is observed that can be detected with high selectivity. With these results, it can be noted that the OOHG-2 electrode holds great hope for detection ovarian cancer by electrochemical methods.
Keywords : MBA, onion oil, electrochemical sensor, CA 125, ovarian cancer