İnhibition of brain glutathione reductase by sodium arsenite

Authors : Nl AÇAN, Ef TEZCAN

Pages : 115-118

View : 20 | Download : 12

Publication Date : 1994-08-01

Article Type : Research Paper

Abstract :The aim of this study is to investigate the inhibition characteristics of brain glutathione reductase insert ignore into journalissuearticles values(GSSGR); under reducing conditions. Sheep brain glutathione reductase insert ignore into journalissuearticles values(GSSGR, NADinsert ignore into journalissuearticles values(P);H: oxidized glutathione oxidoreductase, EC 1.6.4.2); was purified about 1 1000 fold with a method employing ammonium sulphate fractionation, heat denaturation, 2`,5‘-ADP Sepharose 4B and Sepharose C16B chromatography steps. NADPH is an inhibitor of brain GSSGR. Sodium arsenite, which is a specific inhibitor of enzymes having two nascent -SH groups, had little inhibitory effect on GSSGR when incubated with the enzyme alone. When the enzyme was incubated with both NADPH and arsenite, inhibition was intensified. 2-Mercaptoethanol and reduced glutathione enhanced the inhibition further; whereas oxidized glutathione protected the enzyme from inhibition. The reaction mechanism of the enzyme is discussed in the light of these findings.
Keywords : Glutathione reductase, sheep brain, inhibition, NADPH, sodium arsenite, thiol reagents