The neuroprotective effect of lamotrigine against glutamate excitotoxicity in SH-SY5Y human neuroblastoma cells

Authors : Berna TERZIOGLU BEBITOGLU, Elif OGUZ, Nazife Gokce ACET, Ajla HODZIC, Fatime TEMEL, Saniye ADA, Andac KILICKAP

Pages : 146-152

Doi:10.5472/marumj.816319

View : 59 | Download : 10

Publication Date : 2020-10-23

Article Type : Research Paper

Abstract :Objective: Glutamate-induced excitotoxicity has a role in the pathophysiology of neurodegenerative disorders. Lamotrigine, an antiepileptic drug, also used to treat bipolar disorders, may be protective against excitotoxic insult. The aim of the study was to investigate the neuroprotective effect of lamotrigine against the glutamate excitotoxicity in SH-SY5Y cell line. Materials and Methods: SH-SY5Y human neuroblastoma cells were pre-treated with lamotrigine insert ignore into journalissuearticles values(50-100-150 μM); prior to exposure to 15 mM glutamate. The 3-insert ignore into journalissuearticles values(4,5-dimethythiazol – 2-yl);-2,5 – diphenyl tetrazolium bromide insert ignore into journalissuearticles values(MTT); assay was performed to determine cell viability. The anti-oxidant effect of lamotrigine and the role of inflammatory parameters were determined by measuring superoxide dismutase insert ignore into journalissuearticles values(SOD);, hydrogen peroxide insert ignore into journalissuearticles values(H2O2);, IL-1β, IL-6 and TNF-α. Results: Intracellular calcium levels and lactate dehydrogenase insert ignore into journalissuearticles values(LDH); activity increased in glutamate exposed cells. Pre-treatment of cells with MK-801 showed no protective features against glutamate excitotoxicity. Treatment with 100 μM lamotrigine was effective in increasing the viability of glutamate exposed cells and in reducing H2O2 increase in these cells. The SOD activity increased by lamotrigine treated cells exposed to glutamate. IL-1β, IL-6 and TNF-α levels increased after induction with glutamate and attenuated by lamotrigine. Conclusion: Overall, our results confirmed the critical role of inflammation and oxidative stress in glutamate-induced excitotoxicity and lamotrigine may exert a protective effect. Keywords: Lamotrigine, Glutamate excitotoxicity,
Keywords : Lamotrigine, Glutamate excitotoxicity, SH SY5Y, Oxidative stress parameter, Cytokines, MK 801

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