The Protective Effect of Amino-guanidine, an Inducible Nitric Oxide Synthase Inhibitor, on Aluminium Sulphate Neuro-toxicity in the Rat (Wistar albino) Cerebellar Purkinje Cells: Stereological Study

Authors : Burcu DEMİREL YILMAZ, Hayri GENÇ, Bengi BAYSAL, Banu EREN

Pages : 7-14

Doi:10.19127/mbsjohs.322015

View : 60 | Download : 13

Publication Date : 2017-12-28

Article Type : Research Paper

Abstract :Objective: Aluminium insert ignore into journalissuearticles values(Al); is quite abundant in nature and humans are frequently exposed to Al in daily life. Aluminium salts can exist in different forms and they may have toxic impacts on several tissues including brain. In this study, potential preventive effects of amino-guanidine insert ignore into journalissuearticles values(AG); insert ignore into journalissuearticles values(100 mg/kg, i.p.);, an inducible nitric oxide synthase inhibitor, on neuron damage to be created by aluminium sulphate insert ignore into journalissuearticles values(3 mg/kg, i.c.v.); in cerebellar Purkinje cells were determined. Methods: 24 female Wistar albino rats were divided into 4 groups with 6 rats in each: Control insert ignore into journalissuearticles values(C);, Sham insert ignore into journalissuearticles values(S);, Aluminium sulphate insert ignore into journalissuearticles values(Al2insert ignore into journalissuearticles values(SO4);3);, Aluminium sulphate + Amino-guanidine insert ignore into journalissuearticles values(Al2insert ignore into journalissuearticles values(SO4);3+AG);. A single aluminium sulphate insert ignore into journalissuearticles values(3 mg/kg); dose dissolved in 0.9% NaCl was injected intracerebroventricularly to aluminium sulphate and aluminium sulphate + amino-guanidine groups at the beginning of experiments. Following aluminium sulphate injection, amino-guanidine insert ignore into journalissuearticles values(100 mg/kg); dissolved in distilled water was injected to aluminium sulphate + amino-guanidine group intraperiteonally for 15 days. Nothing was administered to control group, a single dose of 0.9% insert ignore into journalissuearticles values(3 mg/kg, i.c.v.); sodium chloride insert ignore into journalissuearticles values(NaCl); was administered to sham group at the beginning of experiments. Cerebellum tissues of the rats were removed 15 days after treatments and they were assessed histopathologically and stereologically. Results: Stereological optic fractionation method revealed cerebellar total number of Purkinje cells as 417615±16238,8 in control group; 378650±20171,6 in Sham group; 272945±15499,5 in Aluminium sulphate group; 324581±16324,8 in Aluminium sulphate + Amino-guanidine group. Conclusion: It was concluded based on present findings that amino-guanidine reduced aluminium induced Purkinje cell loss through nitric oxide synthase insert ignore into journalissuearticles values(NOS); inhibition. 
Keywords : Aluminium, Amino guanidine, Cerebellum, Purkinje cell, Stereology

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