Genomic and Transcriptomic Sequencing and Analysis Approaches

Authors : Ümmügülsüm TANMAN ZIPLAR, Demet CANSARANDUMAN, Mine TÜRKTAŞ

Pages : 34-42

Doi:10.19127/mbsjohs.370490

View : 27 | Download : 18

Publication Date : 2018-04-20

Article Type : Review Paper

Abstract :In this review, we explained that genomic and transcriptomic sequences and analysis assays. First of all, we detailed information of genomic and transcriptomic terms and related analyses. Genomics is aimed to elucidate the structural and functional properties of the genomes. Transcriptomics are used to express quantities of transcripts in a physiological state and specific developmental stage. The methods of genomic and transcriptomic analyses imply highly productive sequence analysis or microarray hybridization analysis as well as bioinformatics analyses. The sequencing technologies include set of methods such as preparing template, sequencing, imaging and data analysis. Firstly, the nucleotide information peculiar to DNA and RNA is obtained by means of the chosen technology in accordance with the goal and scope of the study. The obtained sequences are aligned with respect to a familiar reference sequence, or are combined as de novo . Subsequently, it is determined whether the distinct genomic sets are connected with the other genomic sets by overlapping distinct genomic sets, such as aligned sequence readings, gene annotation, EST, genetic polymorphism, and mobile elements. So, the structural variant to which the obtained sequence data are peculiar is determined. Within the scope of the study, giving information about sequencing technologies and the methods of analyses of the obtained sequences is aimed for researchers work on this subject.
Keywords : Genomics, Transcriptomics, Bioinformatics