INVESTIGATION OF PEROXIDASE-MIMICKING PROPERTIES OF FeNiCo METAL-ORGANIC FRAMEWORK DECORATED MWCNT COMPOSITE NANOMATERIAL

Authors : Yudum Tepeli Büyüksünetçi

Pages : 1-9

Doi:10.22531/muglajsci.1687954

View : 96 | Download : 153

Publication Date : 2025-12-31

Article Type : Research Paper

Abstract :Enzyme-mimicking materials replicate natural enzyme functions, offering more durable, cost-effective, and eco-friendly alternatives, making them important for biotechnology and industrial applications. In this study, the peroxidase like properties of a FeNiCo metal-organic framework-decorated multi-walled carbon nanotube (FeNiCo MOF-MWCNT) composite nanomaterial were investigated for the first time. The nanocomposite was synthesized and characterized using Scanning Electron Microscopy (SEM), SEM-Mapping, SEM-EDS, XRD, and FT-IR techniques. Its enzyme-mimicking activity was evaluated using a colorimetric, non-enzymatic H₂O₂ biosensor with 3,3\\\',5,5\\\'-Tetramethylbenzidine (TMB) as the substrate. The biosensor comprises optimized amounts of FeNiCo MOF-MWCNT and TMB. Upon H₂O₂ addition, the nanocomposite mimics peroxidase activity by reducing H₂O₂ to H₂O, while oxidizing TMB to its blue-colored form, TMB(ox), showing absorbance at 654 nm. After optimizing conditions, the biosensor exhibited a linear response to H₂O₂ concentrations ranging from 0.1 mM to 12 mM. It also demonstrated high selectivity, with negligible interference from substances like KCl, ascorbic acid, urea, and NaCl. Accuracy tests using commercial H₂O₂ disinfectant showed results between 100% and 103%, confirming the biosensor\\\'s reliability for practical applications.
Keywords : Metal organik çerçeveler, enzim taklit, enzimsiz H2O2 biyosensörü, kolorimetrik biyosensör

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