COMPARISON OF THE MICROTITER PLATE METHOD AND THE CONGO RED AGAR TECHNIQUE IN THE DETERMINATION OF STAPHYLOCOCCAL BIOFILM

Authors : Sevda Er

Pages : 128-132

Doi:10.22531/muglajsci.1493167

View : 49 | Download : 84

Publication Date : 2024-06-30

Article Type : Research Paper

Abstract :Coagulase-Negative Staphylococci are opportunistic pathogens that are commensal in human flora. One of the most important virulence factors known in the pathogenesis of infections of these bacteria is biofilm formation. The Microtiter Plate Method and The Congo Red Agar Technique are widely used to reveal biofilm formation. This study aims to compare human coagulase negative Staphylococcus spp. bacterial isolates, biofilm formations with the Microtiter Plate Method and Congo Red Agar Technique. In the study, it was concluded that 35 of 41 human coagulase negative staphylococcal isolates did not form biofilms according to the microtiter plate method, 6 isolates formed a weak biofilm, and none of the isolates formed a biofilm on the Congo Red Agar surface. It has been concluded that the results of the Microtiter Plate Method are more reliable, since the interpretation of the result in the Congo Red Agar Technique is difficult and subjective, based on observation. Since there are very few studies in the literature comparing the biofilm formation of coagulase negative staphylococci with the Microtiter Plate Method and the Congo Red Agar Technique, this study will be among the preliminary studies and will contribute to the literature.
Keywords : Biyofilm, Koagülaz Negatif Stafilokok, Kongo Kırmızısı Agar Tekniği, Mikrotitre Plaka Yöntemi, Vajina