High Frequency Adventitious Shoot Regeneration From Petiole and Leaf Explants of Endemic Astragalus Polemoniacus Bunge

Authors : Semra MİRİCİ

Pages : 31-34

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Publication Date : 2004-12-25

Article Type : Research Paper

Abstract :High frequency adventitious shoot regeneration was obtained by using petioles and leaves of endemic Astragalus pole-moniacus Bunge. Various concentrations of 6-benzylaminopurine insert ignore into journalissuearticles values(BAP);, α–naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); and thidiazuron insert ignore into journalissuearticles values(TDZ); were added to Murashige and Skoog insert ignore into journalissuearticles values(MS); medium. The highest frequency of adventitious shoot regeneration insert ignore into journalissuearticles values(100%); and the highest number of adventitious shoots per explant were obtained from petiole explants on a medium containing 4 mg/l BAP and 0.1 mg/l NAA. The shoots developed in vitro were transferred to hormone-free medium or media supplemented with 0.5, 1 and 2 mg/l NAA for rooting. The best root formation was observed in hormone-free or 2 mg/l NAA containing media. The rooted plantlets were acclimatized in pots covered with plastic bags. Normal chromosome number insert ignore into journalissuearticles values(2n=16); was noted in the root tips of the plantlets.
Keywords : Astragalus polemoniacus Bunge, organogenesis, yaprak sapı, yaprak