Investigation of Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) Polymorphism in Anatolian Black and Holstein Friesian Cattle Breeds

Authors : Marwan FADHIL, İbrahim AYTEKIN, Uğur ZÜLKADİR

Pages : 137-140

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Publication Date : 2020-08-20

Article Type : Research Paper

Abstract :Insulin-like growth factor binding protein-3 insert ignore into journalissuearticles values(IGFBP-3); gene is a structural gene which is associated with development and growth in livestock. The present study aimed to investigate the polymorphisms of IGFBP-3 gene in Holstein Friesian insert ignore into journalissuearticles values(HF); and Anatolian Black insert ignore into journalissuearticles values(AB); cattle breeds. BsuRI insert ignore into journalissuearticles values(GG↓CC); restriction enzyme was used to detect of IGFBP-3 gene polymorphism. Although Anatolian Black breed was monomorphic insert ignore into journalissuearticles values(AA genotype);, three genotypes insert ignore into journalissuearticles values(AA, AB and BB); were detected in Holstein Friesian breed by digestion of PCR products with BsuRI. The A and B allele frequencies were 0.57 and 0.43, respectively, in Holstein Friesian breed, while A allele frequency was1.00 in Anatolian Black breed. AA, AB and BB genotype frequencies were 0.32, 0.50 and 0.18, respectively in the Holstein Friesian breed. All three possible genotypes weare detected in Holstein Friesian breed. In the analysis made taking into account Hardy-Weingberg equilibrium, significant deviation was not observed in terms of genotype distributions insert ignore into journalissuearticles values(P>0.05);. In other words, the Holstein Friesian cattle population was found in the Hardy-Weinberg equilibrium.
Keywords : Anatolian Black, Holstein Friesian, IGFBP 3, PCR RFLP, BsuRI, Polymorphism