The Identification of Genetic Variation in Insulin-Like Growth Factors-I (IGF-I) Gene Region in Some Turkish Shee

Authors : Mustafa TAWFEEQ, İsmail KESKİN, Fatma İLHAN

Pages : 189-192

View : 21 | Download : 18

Publication Date : 2020-12-25

Article Type : Research Paper

Abstract :Insulin-like growth factors insert ignore into journalissuearticles values(IGFs); areknown as peptides with important metabolic effects required for cellular growth and metabolism. IGF-I is synthesized in liver tissue under the control of growth hormone insert ignore into journalissuearticles values(GH); and released to blood. In the process of GH to accelerate growth, IGF-I occupies a large place. IGF-I clearly showed its important effects on the growth of animal studies. In this study, promoter region of the IGF-I gene were amplified by polymerase chain reaction insert ignore into journalissuearticles values(PCR); in sheep breeds insert ignore into journalissuearticles values(Akkaraman, Kivircik, Awassi, Sakiz, Daglıc, Morkaraman, MalyaKarayaka insert ignore into journalissuearticles values(15 to 20 sheep per breed);); reared in Turkey. Informative restriction fragment length polymorphisms insert ignore into journalissuearticles values(RFLPs); were obtained with HaeII enzyme. The digestion of IGF-I gene with HaeII produced two alleles and three genotypes. Genotype frequencies were 59%, 19%and 22% for AA, BB and AB genotypes, respectively. Allele frequencies were 0.70 for A allele and 0.30 for the B allele. This study indicates the genetic profiles of the IGF-1 gene in native Turkish sheep breeds.
Keywords : Growth hormone, Insulin like growth factor IGF, Sheep, Polymerase chain reaction PCR, Restriction fragment length polymorphism insert ignore into journalissueart