Dihaploidization in Squash Genotypes (Cucurbita spp) as a Rootstock Candidates for Cucumber (Cucumis sativus L.) via I

Authors : Ertan Sait KURTAR, Ahmet BALKAYA, Münevver GÖÇMEN, Onur KARAAĞAÇ

Pages : 34-40

View : 27 | Download : 21

Publication Date : 2017-04-28

Article Type : Research Paper

Abstract :The present study was conducted to determine the haploidization efficiency via irradiated pollen technique in some Cucurbita spp. candidates as rootstock. The pollens of 17 rootstocks candidates were irradiated at 150 Gy gamma ray dose with Co60 source and the haploid embryo stimulation was induced pollination with this pollens. The haploidy frequency was highly influenced by genotypes, in total, 23 fruits, 3998 seeds, 233 embryos, 51 plants and 3 haploid plants were derived from “I” genotypes, and these values were 35, 6464, 822, 222 and 24 in “H” genotypes, respectively. Finally, 192 plants were propagated and acclimatized insert ignore into journalissuearticles values(51 of “I” and 141 of “H” genotypes);, and 27 haploid lines were obtained from these plants. The haploidy frequency was 0.07, 1.29 and 0.13 in “I”, 0.37, 2.92 and 0.69 “H” for per 100 seeds, 100 embryos and a fruit, respectively. Dihaploidization was successfully realized by application of 1% colchicine solution to apical meristems of haploid regenerants.
Keywords : Dihaploidizasyon, Işınlanmış polen, Anaç, Cucurbita spp