Early bacterial identification and carbapenemase detection from positive blood culture by mass spectrometry and Blue-Carba test

Authors : María Eugenia CATTANİ, Sandra COGUT, Sara C KAUFMAN

Pages : 154-162

Doi:10.18621/eurj.469655

View : 17 | Download : 11

Publication Date : 2020-03-04

Article Type : Research Paper

Abstract :Objectives: In this study, we evaluated a rapid and simple protocol for direct identification of microorganisms with Matrix-Assisted Laser Desorption/Ionization Time of Flight, Mass Spectrometry insert ignore into journalissuearticles values(MALDI-TOF MS); after a short incubation in solid medium insert ignore into journalissuearticles values(3 to 5h);. Methods: We have examined a total of 1101 positive blood culture bottles from 782 patients. Results: We obtained a correct identification in 1037 insert ignore into journalissuearticles values(94.18%);; 190 insert ignore into journalissuearticles values(98.44%); were Staphylococcus aureus , and 386 insert ignore into journalissuearticles values(98.44%); Enterobacteriaceae . Both are the most frequent etiological agents of sepsis. A total of 1004 bottles were monomicrobial insert ignore into journalissuearticles values(96.81%); and 33 insert ignore into journalissuearticles values(3.18%);, polimicrobial. In the latter we identified at least one species. Two hundred eighty insert ignore into journalissuearticles values(27.00%); organism isolated were considered skin contaminant. Carbapenemase tests were performed with Blue-Carba test in 140 patinas from Gram negative bacilli, we have detected earlier 27 of 29 positive insert ignore into journalissuearticles values(93.10%);. Conclusions: Mass spectrometry by M ALDI-TOF MS is very useful to quickly identify the microbial agent and Blue-Carba contribute to adapt the antibiotic therapy to obtain a correct clinical management of the patient with bacteremia.
Keywords : MALDI TOF MS, rapid identification, blood culture, carbapenemase, short incubation