MORPHOMETRIC EFFECT OF METHANOLONE ENANTHATE ON SCAPULA IN ADOLESCENT RATS

Authors : Mehmet ÖZDEMİR, İbrahim BOZKURT, Sadullah BAHAR, Özgür ÖZDEMİR

Pages : 22-29

Doi:10.22282/ojrs.2020.60

View : 18 | Download : 8

Publication Date : 2020-01-01

Article Type : Research Paper

Abstract :This study was carried out to determine the effect of Methenolone enanthate ME on the doping list on male and female rat scapula during puberty. In this study, three groups ME, FY, K were created for males and females, each containing 8 rats, and a total of 48 rats were used. Methenolone enanthate Primobolan Depot ® Bayer was administered to the experimental groups at a dose of 0.5 mg / kg 0.5 ml, diluted in peanut oil 5 times a week intraperitoneally for 4 weeks. All rats were euthanized under thiopental 40 mg / kg anesthesia after 4 weeks. After necropsy, both scapula bones were dissected and macerated. At the end of this process, the bones were dried in a 37 C oven for 24 hours. Morphometric measurements of right and left scapula bones of male and female rats in each group were opened in the semiautomatic image analysis program imageJ Ver. 1.44, National Institutes of Health . After calibrating the images, the height of the scapula and then the largest distance perpendicular to the height axis were measured by using the freehand line feature of the program Fig 1 . Area measurements of the medial surfaces of the bones were made using the point counting method Başoglu et al, 2007, Akosman and Özdemir, 2010; Bolat et al, 2011 . For this purpose, by using grid function of imagej program plugins-analyze-grid , grids with 2 mm between two points and 4 mm2 of area represented by each point were created. These grids were superimposed on the bone images and the points falling on the bone surface were counted Fig 2 . The number of points obtained was multiplied by the area represented by each point and the bone surface areas of the animals in the groups were calculated separately in mm2.vAs a result, it was found that the effect of ME on rat scapula height, width and surface area was similar to other AAS’s suppressed bone development in male pubers and encouraged bone development in females. The negative effects of the commonly used doping agents on bones in males in the medium and long term have been tried to be demonstrated with rat models.
Keywords : morphometric, rats, Adolescents rats