Quantitative analysis of Medicago lupulina stem and evaluation of antioxidant activity

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Turkish Journal of Biodiversity
Cilt: 8 Sayı: 2
Quantitative analysis of Medicago lupulina stem and evaluation of antioxidant activity

Quantitative analysis of Medicago lupulina stem and evaluation of antioxidant activity

Authors : Ramazan Erenler, İlyas Yıldız, Esma Nur Geçer, İbrahim Hosaflıoğlu

Pages : 74-80

Doi:10.38059/biodiversity.1843090

View : 103 | Download : 121

Publication Date : 2025-12-31

Article Type : Research Paper

Abstract :Plants have gained considerable interest in drug discovery owing to their rich content of bioactive secondary metabolites, which play a critical role in the pharmaceutical industry. A significant proportion of currently used therapeutic agents are derived from naturally occurring biologically active compounds. In this study, the phenolic composition of Medicago lupulina stem was quantified by LC–MS/MS, revealing salicylic acid as the predominant phenolic compound (78.4 µg/g extract). The antioxidant potential of the methanolic stem extract was evaluated using the DPPH and ABTS assays. The extract exhibited notable antioxidant activity, with IC50 values of 14.8 µg/mL and 8.7 µg/mL in the DPPH and ABTS assays, respectively. Identifying bioactive compounds in M. lupulina stems at high concentrations will significantly contribute to studies aimed at their isolation.
Keywords : Kantitatif analiz, Medicago lupulina gövde, antioksidan aktivite

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